Molecular Fluorescent Sensor: a Gateway to Label-free, Real-time Enzymatic Assay and High-throughput Screening
نویسندگان
چکیده
منابع مشابه
Associative chemosensing by fluorescent macrocycle-dye complexes – a versatile enzyme assay platform beyond indicator displacement.
A label-free in situ method to monitor reactions in real time by using fluorescent supramolecular chemosensors based on cucurbit[8]uril is presented. It allows sensing of enzymatic activity, inhibitor and activator screening, and analyte detection with unprecedented versatility and high sensitivity.
متن کاملNew Usage of a Fluorometric Method to Assay Antioxidant Activity in Plant Extracts
In the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (LDCF) to fluorescent dichlorofluoroscein (DCF) was used for detection of antioxidant properties of plant extracts. The rate of the reaction was followed by monitoring the formation of DCF as a function of time. The antioxidant assay was carried out for different concentrations of gallic acid, Salvia ...
متن کاملCapacitance-based assay for real-time monitoring of endocytosis and cell viability.
Label-free cell-based assays have emerged as a promising means for high-throughput screening. Most label-free sensors are based on impedance measurements that reflect the passive electrical properties of cells. Here we introduce a capacitance-based assay that measures the dielectric constant (capacitance) of biological cells, and demonstrate the feasibility of analyzing endocytosis and screenin...
متن کاملHigh-Throughput Screening of Small Molecule Libraries using SAMDI Mass Spectrometry
High-throughput screening is a common strategy used to identify compounds that modulate biochemical activities, but many approaches depend on cumbersome fluorescent reporters or antibodies and often produce false-positive hits. The development of "label-free" assays addresses many of these limitations, but current approaches still lack the throughput needed for applications in drug discovery. T...
متن کاملLabel-free method for anti-glucopeptide antibody detection in Multiple Sclerosis
Surface plasmon resonance technique is particularly interesting in immunology because it has the potential to visualize label-free antigen-antibody interactions in real-time, thus enabling antibody detection and monitoring. Herein we release the guidelines for the correct use of a method to detect specific antibodies directly in Multiple Sclerosis patients' sera using a glucopeptide-based label...
متن کامل